Neural Stem Cells Direct Axon Guidance via Their Radial Fiber Scaffold.

Neural stem cells directly or indirectly generate all neurons and macroglial cells and guide migrating neurons by using a palisade-like scaffold made of their radial fibers. Here, we describe an unexpected role for the radial fiber scaffold in directing corticospinal and other axons at the junction between the striatum and globus pallidus. The maintenance of this scaffold, and consequently axon pathfinding, is dependent on the expression of an atypical RHO-GTPase, RND3/RHOE, together with its binding partner ARHGAP35/P190A, a RHO GTPase-activating protein, in the radial glia-like neural stem cells within the ventricular zone of the medial ganglionic eminence. This role is independent of RND3 and ARHGAP35 expression in corticospinal neurons, where they regulate dendritic spine formation, axon elongation, and pontine midline crossing in a FEZF2-dependent manner. The prevalence of neural stem cell scaffolds and their expression of RND3 and ARHGAP35 suggests that these observations might be broadly relevant for axon guidance and neural circuit formation. Copyright © 2020 Elsevier Inc. All rights reserved.

Citation

Navjot Kaur, Wenqi Han, Zhuo Li, M Pilar Madrigal, Sungbo Shim, Sirisha Pochareddy, Forrest O Gulden, Mingfeng Li, Xuming Xu, Xiaojun Xing, Yutaka Takeo, Zhen Li, Kangrong Lu, Yuka Imamura Kawasawa, Begoña Ballester-Lurbe, Juan Antonio Moreno-Bravo, Alain Chédotal, José Terrado, Ignacio Pérez-Roger, Anthony J Koleske, Nenad Sestan. Neural Stem Cells Direct Axon Guidance via Their Radial Fiber Scaffold. Neuron. 2020 Sep 23;107(6):1197-1211.e9

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PMID: 32707082

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