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The status of T cell receptors (TCRs) repertoire is associated with the occurrence and progress of various diseases and can be used in monitoring the immune responses, predicting the prognosis of disease and other medical fields. High-throughput sequencing promotes the studying in TCR repertoire. The chapter focuses on the whole process of TCR profiling, including DNA extraction, library construction, high-throughput sequencing, and how to analyze data.

Citation

Huixin Lin, Yonggang Peng, Xiangbin Chen, Yuebin Liang, Geng Tian, Jialiang Yang. T Cell Receptor Repertoire Sequencing. Methods in molecular biology (Clifton, N.J.). 2020;2204:3-12

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PMID: 32710310

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