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    We developed a functional lineage tracing tool termed CaTCH (CRISPRa tracing of clones in heterogeneous cell populations). CaTCH combines precise clonal tracing of millions of cells with the ability to retrospectively isolate founding clones alive before and during selection, allowing functional experiments. Using CaTCH, we captured rare clones representing as little as 0.001% of a population and investigated the emergence of resistance to targeted melanoma therapy in vivo.


    Christian Umkehrer, Felix Holstein, Laura Formenti, Julian Jude, Kimon Froussios, Tobias Neumann, Shona M Cronin, Lisa Haas, Jesse J Lipp, Thomas R Burkard, Michaela Fellner, Thomas Wiesner, Johannes Zuber, Anna C Obenauf. Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters. Nature biotechnology. 2021 Feb;39(2):174-178

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    PMID: 32719478

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