Biosynthetic molecular imaging probe for tumor-targeted dual-modal fluorescence/magnetic resonance imaging.

Huaixin Zhao, Huiting Zhao, Yi Jiao, Yi Zhu, Chunxia Liu, Feng Li, Yinsong Wang, Zi Gu, Dayong Yang

Biomaterials 2020 Oct

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Molecular imaging enables noninvasive visualization of cancer-related biological processes for tumor diagnostics; engineering molecular probes for accurate and efficient tumor imaging remains challenge. Herein, by using a biosynthetic strategy, we prepared a tumor-targeted fluorescence/magnetic resonance protein-based probe for dual-modal imaging. The probe was biosynthesized by the fusion of targeting peptide (RGD) with fluorescence protein (RFP) and a small peptide (LBT) that had strong affinity with Gd3+. The probe showed excellent RGD-mediated tumor targeting and stable infrared emission, enabling sensitive tumor-specific imaging. Moreover, efficient T1-weighted magnetic resonance imaging was observed both in vitro and at tumor sites, facilitating high spatial resolution tumor imaging. The simultaneous dual-modal imaging provided more complementary information than each imaging modality. Our work demonstrates the capability of biosynthetic strategy to engineer molecular imaging probes for tumor-targeted multimodal bioimaging, and the probes can be customized to precisely localize and monitor other types of diseases. Copyright © 2020 Elsevier Ltd. All rights reserved.

Citation

Huaixin Zhao, Huiting Zhao, Yi Jiao, Yi Zhu, Chunxia Liu, Feng Li, Yinsong Wang, Zi Gu, Dayong Yang. Biosynthetic molecular imaging probe for tumor-targeted dual-modal fluorescence/magnetic resonance imaging. Biomaterials. 2020 Oct;256:120220