Endothelial Cells: Co-culture Spheroids.

The development and maintenance of a functioning vascular system is a critical function for many aspects of tissue growth and regeneration. Vascular endothelial cell in vitro co-culture spheroids are self-organized cell composites that have the capacity to recapitulate the three-dimensional tissue microenvironment. These spheroid testing platforms aim to better understand the mechanisms of functional tissue and how new therapeutic agents can drive these 3D co-culture processes. Here we describe direct cell-cell 3D endothelial co-culture spheroid methods, to examine the physiological spatial growth and cell-cell interaction of vascular cells and surrounding native tissue cells in the formation of vascular networks within spheroids and the potential to regenerate tissue.

Citation

Janos M Kanczler, Julia A Wells, Richard O C Oreffo. Endothelial Cells: Co-culture Spheroids. Methods in molecular biology (Clifton, N.J.). 2021;2206:47-56

Mesh Tags

PMID: 32754810

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