Comprehensive identification and analysis of DELLA genes throughout the plant kingdom.

DELLAs play key roles in plant gibberellin signaling pathways and are generally important in plant development and growth. However, DELLAs in many plant taxa have not yet been systematically analyzed. In our study, we searched for DELLA genes across 58 green plant genomes and found 181 DELLAs. Structure analysis showed some DELLA domains do not contain "D-E-L-L-A" sequences and instead contain similar domains, including DGLLA and DSLLH domains. "VHYNP" motifs in plant DELLAs comprise 23 types of sequences, while some DELLAs did not contain GRAS domains. In grape, we found that the DELLA protein GSVIVT01015465001 contains an F-box domain, while apple DELLA proteins MDP0000220512 and MDP0000403162 contain a WW domain and a BCIP domain, respectively. These DELLAs can be divided into 22 homologous groups and 17 orthologous groups, and 35 paralogous genes were identified. In total, 35 positively selected genes (PSGs) and 121 negatively selected genes (NSGs) were found among DELLAs based on selective pressure analysis, with an average Ks of NSGs that was significantly higher than that of PSGs (P < 0.05). Among the paralogous groups, CBI and Fop were significantly positively correlated with GC, GC1, GC2, GC12, and GC3, while CAI was significantly positively correlated with GC, GC1, GC12, and GC. The paralogous groups with ω values exceeding 1 had significantly higher Ka values. We also found some paralogous groups with ω values exceeding 1 that differed in their motifs. This study provides helpful insights into the evolution of DELLA genes and offers exciting opportunities for the investigation of DELLA functions in different plants.

Citation

Pengfei Wang, Qianqian Zhang, Yingchun Chen, Yanxia Zhao, Fengshan Ren, Hongmei Shi, Xinying Wu. Comprehensive identification and analysis of DELLA genes throughout the plant kingdom. BMC plant biology. 2020 Aug 06;20(1):372

Mesh Tags

Substances

PMID: 32762652

search → result