Quantity and Quality of Basophil RNA Depend on the RNA Extraction Technique.

Victor G Bechmann, Anette Teo Hansen, Lars K Poulsen, Bettina M Jensen

Methods in molecular biology (Clifton, N.J.) 2020

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Basophils have been suggested to express low quantities of RNA, challenging the study of gene expression within these cells. However, the purification technique employed might have an impact on the quantity and quality of RNA purified from basophils. This chapter describes a method which gives an optimal RNA output using a TRIzol-based method in contrast to a commercial kit.

Citation

Victor G Bechmann, Anette Teo Hansen, Lars K Poulsen, Bettina M Jensen. Quantity and Quality of Basophil RNA Depend on the RNA Extraction Technique. Methods in molecular biology (Clifton, N.J.). 2020;2163:241-245