Superresolution Microscopy of Sphingolipids.

Jan Schlegel, Markus Sauer

Methods in molecular biology (Clifton, N.J.) 2021

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This chapter provides a step-by-step protocol to label and visualize sphingolipids by superresolution microscopy with a special focus on single-molecule localization microscopy by dSTORM. We provide information on custom fluorophore conjugation to raft-associated toxins and antibodies, and a labeling protocol for appropriate sample treatment.