Ryutaro Kotaki, Masaharu Kawashima, Asuka Yamaguchi, Naoto Suzuki, Ryo Koyama-Nasu, Daisuke Ogiya, Kazuki Okuyama, Yuichiro Yamamoto, Masako Takamatsu, Natsumi Kurosaki, Kiyoshi Ando, Akihiko Murata, Masato Ohtsuka, So Nakagawa, Koko Katagiri, Ai Kotani
Scientific reports 2020 Aug 11MicroRNAs (miRNAs), one of small non-coding RNAs, regulate many cell functions through their post-transcriptionally downregulation of target genes. Accumulated studies have revealed that miRNAs are involved in hematopoiesis. In the present study, we investigated effects of miR-669m overexpression on hematopoiesis in mouse in vivo, and found that erythroid differentiation was inhibited by the overexpression. Our bioinformatic analyses showed that candidate targets of miR-669m which are involved in the erythropoiesis inhibition are A-kinase anchoring protein 7 (Akap7) and X-linked Kx blood group (Xk) genes. These two genes were predicted as targets of miR-669m by two different in silico methods and were upregulated in late erythroblasts in a public RNA-seq data, which was confirmed with qPCR. Further, miR-669m suppressed luciferase reporters for 3' untranslated regions of Akap7 and Xk genes, which supports these genes are direct targets of miR-669m. Physiologically, miR-669m was not expressed in the erythroblast. In conclusion, using miR-669m, we found Akap7 and Xk, which may be involved in erythroid differentiation, implying that manipulating these genes could be a therapeutic way for diseases associated with erythropoiesis dysfunction.
Ryutaro Kotaki, Masaharu Kawashima, Asuka Yamaguchi, Naoto Suzuki, Ryo Koyama-Nasu, Daisuke Ogiya, Kazuki Okuyama, Yuichiro Yamamoto, Masako Takamatsu, Natsumi Kurosaki, Kiyoshi Ando, Akihiko Murata, Masato Ohtsuka, So Nakagawa, Koko Katagiri, Ai Kotani. Overexpression of miR-669m inhibits erythroblast differentiation. Scientific reports. 2020 Aug 11;10(1):13554
PMID: 32782283
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