Molecular cloning and tissue distribution of marmoset thiopurine S-methyltransferase.

Shotaro Uehara, Yasuhiro Uno, Hiroshi Yamazaki

Drug metabolism and pharmacokinetics 2020 Oct

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The common marmoset (Callithrix jacchus) is a New World monkey that is increasingly used in pharmacological and toxicological studies. Thiopurine S-methyltransferase (TPMT) plays roles in the metabolism of widely used anticancer and anti-inflammatory drugs. Here, we report the isolation and molecular characterization of marmoset TPMT cDNA, which was found to contain an open-reading frame of 245 amino acids that was approximately 92% identical to its human ortholog. Marmoset TPMT was phylogenetically closer to other primate orthologs than to its pig, dog, rabbit, or rodent orthologs. Among the five marmoset tissue types analyzed, marmoset TPMT mRNA was most abundant in kidney and liver, just as human TPMT is. These results suggest that marmoset and human TPMT are similar at the molecular level. Copyright © 2020 The Japanese Society for the Study of Xenobiotics. Published by Elsevier Ltd. All rights reserved.

Citation

Shotaro Uehara, Yasuhiro Uno, Hiroshi Yamazaki. Molecular cloning and tissue distribution of marmoset thiopurine S-methyltransferase. Drug metabolism and pharmacokinetics. 2020 Oct;35(5):475-478