Cryopreservation without dry ice-induced acidification during sample transport.

Jian Wern Ong, Tristan Minifie, Eric Shen Lin, Hassan Ali Abid, Oi Wah Liew, Tuck Wah Ng

Analytical biochemistry 2020 Nov 01

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Dry ice (solid CO2) remains highly useful when temperature-sensitive biological samples need to be cryogenically transported. CO2 released during the sublimation of dry ice can diffuse through gas permeable receptacle material or any defective seals resulting in potential sample acidification and compromised integrity. In addition, the quality of cryopreservation can be undermined once the dry ice is exhausted. The dry ice carrier design described here has been demonstrated to prevent sublimated CO2 from reaching the samples while maintaining storage temperature below -60 °C for 19 h. It is also equipped with microcontroller-based temperature monitoring for traceability and CO2 gas monitoring for safety. Copyright © 2020 Elsevier Inc. All rights reserved.

Citation

Jian Wern Ong, Tristan Minifie, Eric Shen Lin, Hassan Ali Abid, Oi Wah Liew, Tuck Wah Ng. Cryopreservation without dry ice-induced acidification during sample transport. Analytical biochemistry. 2020 Nov 01;608:113906