Correlation Engine 2.0
Clear Search sequence regions


  • egg yolk (2)
  • livestock (2)
  • nitrogen (1)
  • semen (5)
  • sperm (8)
  • swine (1)
  • tris (1)
  • vapor (1)
  • Sizes of these terms reflect their relevance to your search.

    In modern livestock breeding, cryopreserved semen is routinely used for artificial insemination. Sperm cryopreservation allows for long-term storage of insemination doses and secures reproduction at a desired time point. In order to cryopreserve semen, it needs to be carefully processed to preserve its vital functions after thawing. In this chapter, we describe the processes involved in cryopreservation of bull, stallion, and boar sperm. These include preparation of diluents, dilution of sperm in primary and freezing extender, slow cooling from room temperature to 5 °C, packaging of insemination doses in straws, freezing at a defined cooling rate in liquid nitrogen vapor, cryogenic storage, and thawing. Two-step dilution approaches, with commonly used diluents, are presented, namely, TRIS-egg yolk (TEY) extender for bull sperm, skim milk (INRA-82) extender for stallion sperm, and lactose-egg yolk (LEY) extender for boar sperm. Furthermore, simple methods are presented for cooling and freezing of sperm at defined cooling rates.

    Citation

    Harriëtte Oldenhof, Willem F Wolkers, Harald Sieme. Cryopreservation of Semen from Domestic Livestock: Bovine, Equine, and Porcine Sperm. Methods in molecular biology (Clifton, N.J.). 2021;2180:365-377

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 32797421

    View Full Text