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Northern blotting is a classical technique that allows the detection of specific nucleic acids using radioactive or non-radioactive probes. Normally, nucleic acids are denatured and separated by agarose or polyacrylamide gel electrophoresis and transferred and fixed to a membrane prior to detection. Here, we describe a method to analyze specific RNA in native ribonucleoprotein complexes using blue native PAGE with subsequent northern blotting, crosslinking of RNA onto a suitable membrane, and detection using non-radioactive probes.


Lena Krüßel, Steffen Ostendorp, Anna Ostendorp, Julia Kehr. Detection of RNA in Ribonucleoprotein Complexes by Blue Native Northern Blotting. Methods in molecular biology (Clifton, N.J.). 2021;2170:45-51

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PMID: 32797450

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