A Revised Adaptation of the Smart-Seq2 Protocol for Single-Nematode RNA-Seq.

Dennis Chang, Lorrayne Serra, Dihong Lu, Ali Mortazavi, Adler Dillman

Methods in molecular biology (Clifton, N.J.) 2021

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The advancement of transcriptomic studies in plant parasitic nematodes will greatly benefit from the development of single-nematode RNA-seq methods. Since many plant parasitic nematodes are obligate parasites, it is often difficult to efficiently obtain sufficient amounts of nematodes for transcriptomic studies. Here we have adapted SMART-Seq2 for single-nematode RNA-seq requiring only an individual nematode for a sample replicate. This protocol provides a detailed step-by-step procedure of the RNA-seq workflow starting from lysis of the nematode to quantification of transcripts using a user-friendly online platform.

Citation

Dennis Chang, Lorrayne Serra, Dihong Lu, Ali Mortazavi, Adler Dillman. A Revised Adaptation of the Smart-Seq2 Protocol for Single-Nematode RNA-Seq. Methods in molecular biology (Clifton, N.J.). 2021;2170:79-99