Can Cui, Hui Lin, Wei Pu, Chao Guo, Yan Liu, Xiao-Qiong Pei, Zhong-Liu Wu
Applied biochemistry and biotechnology 2021 JanAsymmetric epoxidation catalyzed with styrene monooxygenase (SMO) is a powerful enzymatic process producing enantiopure styrene epoxide derivatives. To establish a more diversified reservoir of SMOs, a new SMO from Bradyrhizobium sp. ORS 375, named BrSMO, was mined from the database and characterized. BrSMO was constituted of an epoxygenase component of 415 amino acid residues and an NADH-dependent flavin reductase component of 175 residues. BrSMO catalyzed the epoxidation of styrene and 7 more styrene derivatives, yielding the corresponding (S)-epoxides with excellent enantiomeric excesses (95- > 99% ee), with the highest activity achieved for styrene. BrSMO also catalyzed the asymmetric sulfoxidation of 7 sulfides, producing the corresponding (R)-sulfoxides (20-90% ee) with good yields.
Can Cui, Hui Lin, Wei Pu, Chao Guo, Yan Liu, Xiao-Qiong Pei, Zhong-Liu Wu. Asymmetric Epoxidation and Sulfoxidation Catalyzed by a New Styrene Monooxygenase from Bradyrhizobium. Applied biochemistry and biotechnology. 2021 Jan;193(1):65-78
PMID: 32808246
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