Purification of Tannerella forsythia Surface-Layer (S-Layer) Proteins.

Sreedevi Chinthamani, Prasad R Settem, Kiyonobu Honma, Takuma Nakajima, Ashu Sharma

Methods in molecular biology (Clifton, N.J.) 2021

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The objective of this chapter is to provide a detailed purification protocol for the surface-layer (S-layer) glycoproteins of the periodontal pathogen Tannerella forsythia. The procedure involves detergent based solubilization of the bacterial S-layer followed by cesium chloride gradient centrifugation and gel permeation chromatography. The protocol is suitable for the isolation of S-layer glycoproteins from T. forsythia strains with diverse O-glycan structures, and aid in understanding the biochemical basis and the role of protein O-glycosylation in bacterial pathogenesis.

Citation

Sreedevi Chinthamani, Prasad R Settem, Kiyonobu Honma, Takuma Nakajima, Ashu Sharma. Purification of Tannerella forsythia Surface-Layer (S-Layer) Proteins. Methods in molecular biology (Clifton, N.J.). 2021;2210:135-142