Insulin Modulates Myogenesis and Muscle Atrophy Resulting From Skin Scald Burn in Young Male Rats.

Hananiah Tardivo Quintana, Vivianne Izabelle de Araújo Baptista, Mariana Cruz Lazzarin, Hanna Karen Moreira Antunes, Luciana Le Sueur-Maluf, Camila Aparecida Machado de Oliveira, Flavia de Oliveira

The Journal of surgical research 2021 Jan

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Burn injuries (BIs) due to scalding are one of the most common accidents among children. BIs greater than 40% of total body surface area are considered extensive and result in local and systemic response. We sought to assess morphological and myogenic mechanisms through both short- and long-term intensive insulin therapies that affect the skeletal muscle after extensive skin BI in young rats. Wistar rats aged 21 d were distributed into four groups: control (C), control with insulin (C + I), scald burn injury (SI), and SI with insulin (SI + I). The SI groups were submitted to a 45% total body surface area burn, and the C + I and SI + I groups received insulin (5 UI/Kg/d) for 4 or 14 d. Glucose tolerance and the homeostatic model assessment of insulin resistance index were determined. Gastrocnemius muscles were analyzed for histopathological, morphometric, and immunohistochemical myogenic parameters (Pax7, MyoD, and MyoG); in addition, the expression of genes related to muscle atrophy (MuRF1 and MAFbx) and its regulation (IGF-1) were also assessed. Short-term treatment with insulin favored muscle regeneration by primary myogenesis and decreased muscle atrophy in animals with BIs, whereas the long-term treatment modulated myogenesis by increasing the MyoD protein. Both treatments improved histopathological parameters and secondary myogenesis by increasing the MyoG protein. Treatment with insulin benefits myogenic parameters during regeneration and modulates MuRF1, an important mediator of muscle atrophy. Published by Elsevier Inc.

Citation

Hananiah Tardivo Quintana, Vivianne Izabelle de Araújo Baptista, Mariana Cruz Lazzarin, Hanna Karen Moreira Antunes, Luciana Le Sueur-Maluf, Camila Aparecida Machado de Oliveira, Flavia de Oliveira. Insulin Modulates Myogenesis and Muscle Atrophy Resulting From Skin Scald Burn in Young Male Rats. The Journal of surgical research. 2021 Jan;257:56-68