The Laetiporus polyketide synthase LpaA produces a series of antifungal polyenes.

Paula Sophie Seibold, Claudius Lenz, Markus Gressler, Dirk Hoffmeister

The Journal of antibiotics 2020 Oct

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The conspicuous bright golden to orange-reddish coloration of species of the basidiomycete genus Laetiporus is a hallmark feature of their fruiting bodies, known among mushroom hunters as the "chicken of the woods". This report describes the identification of an eight-domain mono-modular highly reducing polyketide synthase as sole enzyme necessary for laetiporic acid biosynthesis. Heterologous pathway reconstitution in both Aspergillus nidulans and Aspergillus niger verified that LpaA functions as a multi-chain length polyene synthase, which produces a cocktail of laetiporic acids with a methyl-branched C26-C32 main chain. Laetiporic acids show a marked antifungal activity on Aspergillus protoplasts. Given the multiple products of a single biosynthesis enzyme, our work underscores the diversity-oriented character of basidiomycete natural product biosynthesis.

Citation

Paula Sophie Seibold, Claudius Lenz, Markus Gressler, Dirk Hoffmeister. The Laetiporus polyketide synthase LpaA produces a series of antifungal polyenes. The Journal of antibiotics. 2020 Oct;73(10):711-720