Establishment and Characterization of Monoclonal Antibody Against Canine CD8 Alpha.

Osamu Sakai, Tatsuhito Ii, Kazuyuki Uchida, Masaya Igase, Takuya Mizuno

Monoclonal antibodies in immunodiagnosis and immunotherapy 2020 Aug

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Understanding of the microenvironment of cancer plays a crucial role in cancer research. A tool is needed to evaluate the immune cells surrounding the cancer cells. This study establishes and evaluates a novel monoclonal antibody against canine CD8α (cCD8α). The antibody was produced by immunization of rats with cCD8α-expressing cells. After establishment and selection of hybridoma cells, the clone F3-B2 was established. The reactivity of F3-B2 was confirmed using cCD8α-overexpressing murine cells. Flow cytometric analysis also demonstrated that F3-B2 reacts with cCD8α naturally expressed in canine peripheral blood mononuclear cells and a canine T cell lymphoma cell line. The specimens of lymphoid tissue showed immunohistochemical staining for F3-B2. Moreover, we also found that F3-B2 exhibited reactivity against feline CD8. Thus, this antibody provides a good research tool to analyze CD8-positive cytotoxic lymphocytes in canine and feline tumors.

Citation

Osamu Sakai, Tatsuhito Ii, Kazuyuki Uchida, Masaya Igase, Takuya Mizuno. Establishment and Characterization of Monoclonal Antibody Against Canine CD8 Alpha. Monoclonal antibodies in immunodiagnosis and immunotherapy. 2020 Aug;39(4):129-134