A highly selective fluorescent probe for the intracellular measurement of magnesium ion.

Deven Patel, Ruogu Peng, Haitao Guo, Raechel Lin, Qin Zhao, Jinfang Liao, Zhenjun Diwu

Analytical biochemistry 2020 Nov 15

filter terms:

Magnesium ion (Mg+2) plays an important role in various biological processes. All the commercial indicators available share a common drawback, i.e., they have a higher affinity towards calcium ions (Ca+2) than Mg+2. In this study, we reported a new robust green fluorescent indicator, Mag-520, for detection of Mg+2 in live cells. Our results showed that Mag-520 has 10 fold higher affinity towards Mg+2 than Ca+2, while mag-fluo-4 has less than 0.5 fold affinity to Mg+2 than Ca+2 under the same conditions using flow cytometry and fluorescence microscopy. The results demonstrated that Mag-520 provides a better tool to measure Mg+2 with less interference from Ca+2. Copyright © 2020 Elsevier Inc. All rights reserved.

Citation

Deven Patel, Ruogu Peng, Haitao Guo, Raechel Lin, Qin Zhao, Jinfang Liao, Zhenjun Diwu. A highly selective fluorescent probe for the intracellular measurement of magnesium ion. Analytical biochemistry. 2020 Nov 15;609:113910