Bacillus amyloliquefaciens PDR1 from root of karst adaptive plant enhances Arabidopsis thaliana resistance to alkaline stress through modulation of plasma membrane H+-ATPase activity.

Fei Li, Tianlong Shi, Xiaoxin Tang, Ming Tang, Jiyi Gong, Yin Yi

Plant physiology and biochemistry : PPB 2020 Oct

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Exploration of native microbes is a feasible way to develop microbial agents for ecological restoration. This study was aimed to explore the impact of Bacillus amyloliquefaciens PDR1 from karst adaptive plant on the activity of root plasma membrane H+-ATPase in Arabidopsis thaliana. A. thaliana was cultured in presence or absence of B. amyloliquefaciens PDR1 and its effects on the growth were evaluated by measuring the taproot length and dry weight. The rhizosphere acidification capacity was detected by a pH indicator, a pH meter and non-invasive micro-test techniques (NMT). The nutrient uptake was performed using appropriate methods. A combination of transcriptome sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) was used to measure the expression of functional genes that regulate the plasma membrane H+-ATPase activity in A. thaliana roots. Functional analysis was performed to understand how B. amyloliquefaciens regulates biological processes and metabolic pathways to strengthen A. thaliana resistance to alkaline stress. Here, we show that volatile organic compounds (VOCs) from B. amyloliquefaciens PDR1 promoted the growth and development of A. thaliana, enhanced the plasma membrane H+-ATPase activity, and affected ion absorption in Arabidopsis roots. Moreover, B. amyloliquefaciens PDR1 VOCs did not affect the expression of the gene coding for plasma membrane H+-ATPase, but affected the expression of genes regulating the activity of plasma membrane H+-ATPase. Our findings illuminate the mechanism by which B. amyloliquefaciens regulates the growth and alkaline stress resistance of A. thaliana, and lay a foundation for wide and efficient application for agricultural production and ecological protection. Copyright © 2020 Elsevier Masson SAS. All rights reserved.

Citation

Fei Li, Tianlong Shi, Xiaoxin Tang, Ming Tang, Jiyi Gong, Yin Yi. Bacillus amyloliquefaciens PDR1 from root of karst adaptive plant enhances Arabidopsis thaliana resistance to alkaline stress through modulation of plasma membrane H+-ATPase activity. Plant physiology and biochemistry : PPB. 2020 Oct;155:472-482