An efficient protocol for the preparation of linear arabino-oligosaccharides.

Huimin Shi, Jialei Xu, Wenqing Wang, Mengdi Jia, Yifa Zhou, Lin Sun

Carbohydrate research 2020 Oct

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In this study, we report the development of an efficient protocol in the preparation of linear arabino-oligosaccharides derived from sugar beet arabinan. By optimizing hydrolytic conditions and separation on tandem Bio-Gel P4 columns, we obtained arabino-oligosaccharides with various degrees of polymerization (DP) from 2 to 15. All of these α-1,5-linked arabino-oligosaccharides are highly pure (>95%) as determined by HPAEC, MALDI-TOF mass spectrometry and 13C NMR spectroscopy. Due to their purity, these oligosaccharides can be used as standards to identify other oligosaccharides and as substrates to characterize new arabinan-specific enzymes, as well as for the development of new functional oligosaccharides. Copyright © 2020 Elsevier Ltd. All rights reserved.

Citation

Huimin Shi, Jialei Xu, Wenqing Wang, Mengdi Jia, Yifa Zhou, Lin Sun. An efficient protocol for the preparation of linear arabino-oligosaccharides. Carbohydrate research. 2020 Oct;496:108131