AKAP350 enables p150glued /EB1 interaction at the spindle poles.

A-kinase anchoring protein 350 (AKAP350) is a centrosomal/Golgi scaffold protein, critical for the regulation of microtubule dynamics. AKAP350 recruits end-binding protein 1 (EB1) to the centrosome in mitotic cells, ensuring proper spindle orientation in epithelial cells. AKAP350 also interacts with p150glued, the main component of the dynactin complex. In the present work, we found that AKAP350 localized p150glued to the spindle poles, facilitating p150glued/EB1 interaction at these structures. Our results further showed that the decrease in AKAP350 expression reduced p150glued localization at astral microtubules and impaired the elongation of astral microtubules during anaphase. Overall, this study provides mechanistic data on how microtubule regulatory proteins gather to define microtubule dynamics in mitotic cells. Copyright © 2020 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

Citation

Evangelina Almada, Alejandro Pariani, Tomás Rivabella Maknis, Florencia Hidalgo, Rodrigo Vena, Cristián Favre, M Cecilia Larocca. AKAP350 enables p150glued /EB1 interaction at the spindle poles. Biochimie. 2020 Oct;177:127-131

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PMID: 32841682

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