Catalysis by protein acetyltransferase Gcn5.

Brittany N Albaugh, John M Denu

Biochimica et biophysica acta. Gene regulatory mechanisms 2021 Feb

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Gcn5 serves as the defining member of the Gcn5-related N-acetyltransferase (GNAT) superfamily of proteins that display a common structural fold and catalytic mechanism involving the transfer of the acyl-group, primarily acetyl-, from CoA to an acceptor nucleophile. In the case of Gcn5, the target is the ε-amino group of lysine primarily on histones. Over the years, studies on Gcn5 structure-function have often formed the basis by which we understand the complex activities and regulation of the entire protein acetyltransferase family. It is now appreciated that protein acetylation occurs on thousands of proteins and can reversibly regulate the function of many cellular processes. In this review, we provide an overview of our fundamental understanding of catalysis, regulation of activity and substrate selection, and inhibitor development for this archetypal acetyltransferase. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Brittany N Albaugh, John M Denu. Catalysis by protein acetyltransferase Gcn5. Biochimica et biophysica acta. Gene regulatory mechanisms. 2021 Feb;1864(2):194627