A dicyanomethylene-4H-pyran-based fluorescence probe with high selectivity and sensitivity for detecting copper (II) and its bioimaging in living cells and tissue.

Zheng Li, Yanqi Xu, Huandi Xu, Mengyuan Cui, Tianguang Liu, Xiangyu Ren, Jianfei Sun, Dawei Deng, Yueqing Gu, Peng Wang

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy 2021 Jan 05

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Copper (Cu) plays a significant role in the process of oxygenic photosynthesis in living systems. The detection of copper ion (Cu2+) is valuable and meaningful for further investigating the functions of Cu2+ under physiological and pathological conditions. In this paper, a novel fluorescence probe DCM-Cu based on the near-infrared (NIR) fluorophore dicyanomethylene-4H-pyran (DCM) was designed for Cu2+ detection. The probe DCM-Cu possessed characteristic of "turn-on" fluorescent signal in the presence of Cu2+ through the enhanced ICT process. It exhibited satisfactory sensitivity and selectivity toward Cu2+. A good linear correlation was observed between the concentrations of Cu2+ and the fluorescence intensities at 700 nm. The detection limit (LOD) of DCM-Cu toward Cu2+ was calculated to be 2.54 × 10-8 M. Importantly, DCM-Cu was successfully applied in the detection of Cu2+ in living MCF-7 cells and tumor tissue with low cytotoxicity. Therefore, this probe would have the potential to monitor cellular Cu2+ in the living system and be applied to the diagnosis of related diseases. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Zheng Li, Yanqi Xu, Huandi Xu, Mengyuan Cui, Tianguang Liu, Xiangyu Ren, Jianfei Sun, Dawei Deng, Yueqing Gu, Peng Wang. A dicyanomethylene-4H-pyran-based fluorescence probe with high selectivity and sensitivity for detecting copper (II) and its bioimaging in living cells and tissue. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 2021 Jan 05;244:118819