Novel metal-organic framework combining with restricted access molecularly imprinted nanomaterials for solid-phase extraction of gatifloxacin from bovine serum.

Novel restricted access molecularly imprinted nanomaterials (RAMIPs) were successfully prepared on the surface of metal-organic frameworks (NH2-MIL-125) by surface-initiated atom transfer radical polymerization (SI-ATRP) technology. Then it was applied as a solid phase extraction (SPE) material in analysis of quinolones in bovine serum by HPLC detection. NH2-MIL-125@RAMIPs was empolyed as a sorbent for gatifloxacin (GTFX) and the resulted material has a good binding amounts (86.1 mg g-1), rapid binding kinetic (36 min). The results indicated that the prepared NH2-MIL-125@RAMIPs possess excellent specific recognition for GTFX. Combined with high-performance liquid chromatography (HPLC), the SPE column filled with NH2-MIL-125@RAMIPs was applied to selectively enrich GTFX antibiotic from bovine serum. The recovery of GTFX is between 96.8% and 105.6%, with relative standard deviations of 1.7-3.2% (n = 3). The research results illustrate that the method is successfully applied to the selective enrichment of GTFX in bovine serum. It provides a simple and efficient method for the direct detection of GTFX in bovine serum. Copyright © 2020 Elsevier B.V. All rights reserved.

Citation

Shanwen Zhao, Zhian Sun, Xiaoxiao Wang, Jianmin Li, Yanqiang Zhou, Bolin Gong. Novel metal-organic framework combining with restricted access molecularly imprinted nanomaterials for solid-phase extraction of gatifloxacin from bovine serum. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2020 Nov 10;1157:122338

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PMID: 32866919

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