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To investigate the gene expression profile of peripheral blood mononuclear cells (PBMCs) from head and neck squamous cell carcinoma (HNSCC), including oral cancer (OC) and oropharyngeal cancer (OPC) patients, and compare them with healthy controls (HC). Transcriptomic analysis of PBMCs was performed by RNA-sequencing. The upregulated candidate genes were selected for validation by quantitative real-time polymerase chain reaction (qPCR). In addition, related plasma protein levels were determined by enzyme-linked immunosorbent assay (ELISA). Three significantly upregulated genes, including high mobility group nucleosomal binding domain 2 (HMGN2), folate receptor gamma (FOLR3), and amphiregulin (AREG), were selected. In the first cohort, the results showed that only HMGN2 expression was significantly increased in OC patients. In the larger sample size, the overall results demonstrated that HMGN2 expression had a tendency to increase in both OC and OPC patients compared with HC. Interestingly, the plasma HMGN2 (HMG-17) protein level exhibited the same trend as that observed at the transcriptional level. HMGN2 expression and plasma HMG-17 (HMGN2 protein) were increased in both cancer patients compared with HC. This gene may be important for further functional studies in the PBMCs of HNSCC patients. © 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.


Fardeela Bin-Alee, Areeya Arayataweegool, Supranee Buranapraditkun, Patnarin Mahattanasakul, Napadon Tangjaturonrasme, Nattiya Hirankarn, Apiwat Mutirangura, Nakarin Kitkumthorn. Transcriptomic analysis of peripheral blood mononuclear cells in head and neck squamous cell carcinoma patients. Oral diseases. 2021 Sep;27(6):1394-1402

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PMID: 32892371

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