Generation of Random luxCDABE Transcriptional Fusions in the Genome of Salmonella enterica.

Juan J de la Rosa-Altura, Barbora Benesova, Concepción Panadero-Medianero, Aida Amador-Álvarez, Julia Aguilera-Herce, Juan Luis Araujo-Garrido, Joaquín Bernal-Bayard, Francisco Ramos-Morales

Methods in molecular biology (Clifton, N.J.) 2021

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genes (3)

operon (3)

photorhabdus (1)

photorhabdus luminescens (1)

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The luxCDABE operon of Photorhabdus luminescens can be used as a bioluminescent reporter to measure gene transcription nondestructively. Here we describe protocols to (1) generate random transcriptional fusions of the lux operon to genes of the Salmonella genome, (2) screen for specific fusions with constitutive expression, Salmonella pathogenicity island 1-related expression, or Salmonella pathogenicity island 2-related expression, and (3) determine the site of luxCDABE integration.

Citation

Juan J de la Rosa-Altura, Barbora Benesova, Concepción Panadero-Medianero, Aida Amador-Álvarez, Julia Aguilera-Herce, Juan Luis Araujo-Garrido, Joaquín Bernal-Bayard, Francisco Ramos-Morales. Generation of Random luxCDABE Transcriptional Fusions in the Genome of Salmonella enterica. Methods in molecular biology (Clifton, N.J.). 2021;2182:141-151