Effects of Euphorbia umbellata extracts on complement activation and chemotaxis of neutrophils.

The species Euphorbia umbellata (leitosinha) has been traditionally used for the treatment of inflammatory diseases and cancer. Evaluation the effect of E. umbellata latex extracts obtained with hexane, chloroform, ethyl acetate and methanol on the activation of the complement pathways and neutrophil chemotaxis. The latex was partitioned using Soxhlet apparatus and hexane, chloroform, ethyl acetate and methanol as solvents. The classical and alternative pathway activity were performed by hemolytic assays with sensitized sheep or rabbit erythrocytes, respectively; the lectin pathway activity was quantified by ELISA, through the measurement of C4 molecules and the chemotaxis of human neutrophils was performed using 1% casein as the chemotactic inducer and Boyden's chamber. GC-Q-ToF and NMR analyses were applied to evaluate the chemical composition of E. umbellata latex extracts. All E. umbellata latex extracts exhibited an inhibitory effect on the activation of the alternative pathway. Methanol and ethyl acetate extracts inhibited the classical pathway while chloroform extract activated this pathway. Ethyl acetate and hexane extracts inhibited lectin activation. All E. umbellata extracts inhibited casein-induced neutrophil chemotaxis. Terpenes and phenolic compounds have been suggested to be present in the E. umbellta latex extracts. The E. umbellata latex was able to modulate the functions of the immune system. Thus, it is possible to infer that the terpenes and phenolic compounds of the phytocomplex of E. umbellata latex can contribute for the activity on the complement pathways. Copyright © 2020 Elsevier B.V. All rights reserved.

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Thais Latansio de Oliveira, Pâmela Dias Fontana, Lorena Bavia, Luiza Stolz Cruz, Amanda Rabello Crisma, Guilherme Lanzi Sassaki, Leociley Rocha Alencar Menezes, Mei Wang, Flávio Luís Beltrame, Iara Jose Messias-Reason. Effects of Euphorbia umbellata extracts on complement activation and chemotaxis of neutrophils. Journal of ethnopharmacology. 2021 Jan 30;265:113348

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PMID: 32896626

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