Rapid Determination of Sufentanil in Human Plasma by UHPLC-QqQ-MS-MS.

Marcin Zawadzki, Grzegorz Kowalski, Agnieszka Chłopaś-Konowałek, Marta Siczek, Małgorzata Sobieszczańska, Wojciech Leppert, Katarzyna Wieczorowska-Tobis, Paweł Szpot

Journal of analytical toxicology 2021 Jul 10

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This paper presents a rapid, sensitive and precise method developed and validated for the quantification of sufentanil in biological samples using ultra-performance liquid chromatography coupled with QqQ-MS-MS. Plasma samples were extracted with simple and fast liquid-liquid extraction (ethyl acetate, pH 9). Calibration curve showed linearity in the concentration range of 0.005-30 µg/L. The lower limit of quantification was 0.010 µg/L. The most important method features are low lower limit of quantification value, simple plasma extraction and small sample volume. This method is suitable not only for evaluation of the pharmacokinetics, toxicology, bioavailability and clinical pharmacology of sufentanil but also for the detection and identification of this compound in human plasma samples for forensic purposes. © The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

Citation

Marcin Zawadzki, Grzegorz Kowalski, Agnieszka Chłopaś-Konowałek, Marta Siczek, Małgorzata Sobieszczańska, Wojciech Leppert, Katarzyna Wieczorowska-Tobis, Paweł Szpot. Rapid Determination of Sufentanil in Human Plasma by UHPLC-QqQ-MS-MS. Journal of analytical toxicology. 2021 Jul 10;45(6):605-611