Dynamics of the RNA polymerase I TFIIF/TFIIE-like subcomplex: a mini-review.

Bruce A Knutson, Rachel McNamar, Lawrence I Rothblum

Biochemical Society transactions 2020 Oct 30

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RNA polymerase I (Pol I) is the most specialized eukaryotic Pol. It is only responsible for the synthesis of pre-ribosomal RNA (rRNA), the precursor of 18S, 5.8S and 28S rRNA, the most abundant cellular RNA types. Aberrant Pol I transcription is observed in a wide variety of cancers and its down-regulation is associated with several genetic disorders. The regulation and mechanism of Pol I transcription is increasing in clarity given the numerous high-resolution Pol I structures that have helped bridge seminal genetic and biochemical findings in the field. Here, we review the multifunctional roles of an important TFIIF- and TFIIE-like subcomplex composed of the Pol I subunits A34.5 and A49 in yeast, and PAF49 and PAF53 in mammals. Recent analyses have revealed a dynamic interplay between this subcomplex at nearly every step of the Pol I transcription cycle in addition to new roles in chromatin traversal and the existence of a new helix-turn-helix (HTH) within the A49/PAF53 linker domain that expands its dynamic functions during the Pol I transcription process. © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society.

Citation

Bruce A Knutson, Rachel McNamar, Lawrence I Rothblum. Dynamics of the RNA polymerase I TFIIF/TFIIE-like subcomplex: a mini-review. Biochemical Society transactions. 2020 Oct 30;48(5):1917-1927