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Optogenetics uses light to manipulate protein localization or activity from subcellular to supra-cellular level with unprecedented spatiotemporal resolution. We used it to control the activity of the Cdc42 Rho GTPase, a major regulator of actin polymerization and cell polarity. In this chapter, we describe how to trigger and guide cell migration using optogenetics as a way to mimic EMT in an artificial yet highly controllable fashion.

Citation

Leo Valon, Simon de Beco. Control of Cell Migration Using Optogenetics. Methods in molecular biology (Clifton, N.J.). 2021;2179:415-425

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PMID: 32939735

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