Strategies for the expression and characterization of artificial myoglobin-based carbene transferases.

Daniela M Carminati, Eric J Moore, Rudi Fasan

Methods in enzymology 2020

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Myoglobin has recently emerged as a versatile metalloprotein scaffold for the design of efficient and selective biocatalysts for abiological carbene transfer reactions, including asymmetric cyclopropanation reactions. Over the past few years, our group has explored several strategies to modulate the carbene transfer reactivity of myoglobin-based catalysts, including the substitution of the native heme cofactor and conserved histidine axial ligand with non-native porphynoid ligands and alternative natural and unnatural amino acids as the metal-coordinating ligands, respectively. Herein, we report protocols for the generation and reconstitution in vitro and in vivo of myoglobin-based artificial carbene transferases incorporating non-native iron-porphynoid cofactors, also in combination with unnatural amino acids as the proximal ligand. These strategies are effective for imparting these myoglobin-based cyclopropanation biocatalysts with altered and improved function, including tolerance to aerobic conditions and improved reactivity toward electrondeficient olefins. © 2020 Elsevier Inc. All rights reserved.

Citation

Daniela M Carminati, Eric J Moore, Rudi Fasan. Strategies for the expression and characterization of artificial myoglobin-based carbene transferases. Methods in enzymology. 2020;644:35-61