Chromatin Profiling in Mouse Embryonic Germ Cells by CUT&RUN.

Srinivasa Abishek Prakash, Joan Barau

Methods in molecular biology (Clifton, N.J.) 2021

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Cleavage under targets and release using nuclease (CUT&RUN) allows the chromatin profiling of proteins of interest for which specific antibodies are available. Because it is performed on intact chromatin in situ, CUT&RUN provides exceptional signal over background, making it an ideal choice for chromatin profiling on primary cells available at limited numbers. Here, we describe its application to the profiling of histone post-translational modifications in germ cells isolated from mouse embryos from 12.5 up to 18.5 days postfertilization. This approach can be applied to as low as 100 isolated germ cells, allowing the generation of multiple genome-wide profiles from the cells obtained from a single embryo.

Citation

Srinivasa Abishek Prakash, Joan Barau. Chromatin Profiling in Mouse Embryonic Germ Cells by CUT&RUN. Methods in molecular biology (Clifton, N.J.). 2021;2214:253-264