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    The removal of lignin is important to the recovery of saccharides from the pre-hydrolysis liquor (PHL) in kraft-based dissolved pulp production. A one-step process for lignin removal from PHL via treatment with horseradish peroxidase (HRP) in the presence of Ca2+ was proposed, and its principle was studied. The results demonstrated synergy between HRP and Ca2+ in lignin removal from PHL, whereas NH4+ had little effect on lignin removal. HRP treatment in the presence of 60 mmol/L of Ca2+ resulted in a lignin removal of 64.8% accompanied by a saccharide loss of 14.2%. HRP catalyzed both the polymerization and depolymerization of the lignin in the PHL. The HRP-catalyzed lignin polymerization rendered some lignin insoluble enabling it to be directly removed. The HRP-catalyzed depolymerization of lignin decreased its molecular weight with an evident increase in its carboxyl content. The insoluble complexes formed between the lignin with carboxyl and the Ca2+ facilitated the removal of the depolymerized lignin. Copyright © 2020 Elsevier B.V. All rights reserved.


    Fusheng Wang, Qin Liu, Jiansong Chen, Zongquan Li, Yingjuan Fu, Menghua Qin. Enhancement of lignin removal from pre-hydrolysis liquor for saccharide recovery via horseradish peroxidase treatment in the presence of Ca2. International journal of biological macromolecules. 2020 Nov 15;163:1989-1994

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    PMID: 32946940

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