The Relevance of SNPs at 3'UTR Region of CASP7 and miR-371b-5p Associated Diseases: A Computational Analysis.

Muhammad Safdar, Sana Zaheer, Rozhgar A Khailany, Sadaf Parvez, Muhammad Naveed, Piplu Bhuiyan, Mehmet Ozaslan, Rebaz Moatasam, Mustafa S Al-Attar, Musarrat Abbas Khan, Yasmeen Junejo

Cell biochemistry and biophysics 2020 Dec

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The process of genetically programmed cell death, or apoptosis, plays a crucialrolein cellular homeostasis and gene expression. Disruption of apoptosis may lead to aberrant immune responses, cancer, and neurodegenerative diseases. Single nucleotide polymorphisms (SNPs) present in various microRNA (miRNA) genes and targets being an alteration of miRNA activity resulting in human diseases. Evidence reported that SNPs increase/decrease the effectiveness of the interaction between miRNAs and their target genes associated with diseases. The primary purpose of this study is not only to identify miRSNPs on the CASP7 gene (caspase-7) and SNPs in miRNA genes targeting 3'UTR but also to evaluate the effect of thesegene variations in apoptosis and their associated diseases. We detected 120 miRNAs binding sites and 27 different SNPs in binding sites of miRNA in 3'UTR of the CASP7 gene by ten different online softwares. Interestingly, miR-371b-5p's binding site on CASP7 has an SNP (rs576198588, G/T) on CASP7 3'UTR, and its genomic sequence has an SNP (rs751339395, G/T) at the same nucleotide with rs576198588. Similarly, two other SNPs (rs774879764, C/G rs750389063, C/T) were identified at the first position binding site of miR-371b-5p. Here, miRSNP (rs576198588) at CASP7 3'UTR and SNP (rs751339395) at miR-371b-5p genomic sequence cross-matches at the same site of binding region. Besides, miR-371b-5p targets many apoptosis-related genes (HIP1, TRIAP1, GSKIP, NIN, DAP, CAAP1, XIAP, TMBIM1, TMBIM4, TNFRSF10A, RAD21, AKT1, BAG1, BAG4) even though it had no apoptosis correlated interaction demonstrated formerly. It assures that CASP7 could have a significant consequence on apoptosis through different pathways. Henceforth, this study was representing and signifying an influential connotation among miR-371b-5p and apoptosis via computational exploration and recommended to have better insight.

Citation

Muhammad Safdar, Sana Zaheer, Rozhgar A Khailany, Sadaf Parvez, Muhammad Naveed, Piplu Bhuiyan, Mehmet Ozaslan, Rebaz Moatasam, Mustafa S Al-Attar, Musarrat Abbas Khan, Yasmeen Junejo. The Relevance of SNPs at 3'UTR Region of CASP7 and miR-371b-5p Associated Diseases: A Computational Analysis. Cell biochemistry and biophysics. 2020 Dec;78(4):541-557