Correlation Engine 2.0
Clear Search sequence regions

  • cofactor (5)
  • cofactor- enzymes (4)
  • nad (4)
  • phosphate (1)
  • research (1)
  • Sizes of these terms reflect their relevance to your search.

    Nicotinamide cofactors enable oxidoreductases to catalyze a myriad of important reactions in biomanufacturing. Decades of research has focused on optimizing enzymes which utilize natural nicotinamide cofactors, namely nicotinamide adenine dinucleotide (phosphate) (NAD(P)+). Recent findings reignite the interest in engineering enzymes to utilize noncanonical cofactors, the mimetics of NAD+ (mNADs), which exhibit superior industrial properties in vitro and enable specific electron delivery in vivo. We compare recent advances in engineering natural versus noncanonical cofactor-utilizing enzymes, discuss design principles discovered, and survey emerging high-throughput platforms beyond the traditional 96-well plate-based methods. Obtaining mNAD-dependent enzymes remains challenging with a limited toolkit. To this end, we highlight design principles and technologies which can potentially be translated from engineering natural to noncanonical cofactor-dependent enzymes. Copyright © 2020 Elsevier Ltd. All rights reserved.


    Edward King, Sarah Maxel, Han Li. Engineering natural and noncanonical nicotinamide cofactor-dependent enzymes: design principles and technology development. Current opinion in biotechnology. 2020 Dec;66:217-226

    Expand section icon Mesh Tags

    Expand section icon Substances

    PMID: 32956903

    View Full Text