Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers.

Hui Qian, Yimei Jia, Michael J McCluskie

Methods in molecular biology (Clifton, N.J.) 2021

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Cryogenic transmission electron microscopy (Cryo-TEM) enables visualizing the physicochemical structure of nanocarriers in solution. Here, we demonstrate the typical applications of Cryo-TEM in characterizing archaeosome-based vesicles as antigen carriers, including the morphology and size of vaccine carriers. Cryo-TEM tomography, incorporated with immunogold labeling for identifying and localizing the antigens, reveals the antigen distribution within archaeosomes in three dimensions (3D).

Citation

Hui Qian, Yimei Jia, Michael J McCluskie. Application of Cryogenic Transmission Electron Microscopy for Evaluation of Vaccine Delivery Carriers. Methods in molecular biology (Clifton, N.J.). 2021;2183:499-511