Protein-induced B-Z transition of DNA duplex containing a 2'-OMe guanosine.

Ho-Seong Jin, Na-Hyun Kim, Seo-Ree Choi, Kwang-Im Oh, Joon-Hwa Lee

Biochemical and biophysical research communications 2020 Dec 10

filter terms:

Structural transformation of the canonical right-handed helix, B-DNA, to the non-canonical left-handed helix, Z-DNA, can be induced by the Zα domain of the human RNA editing enzyme ADAR1 (hZαADAR1). To characterize the site-specific preferences of binding and structural changes in DNA containing the 2'-O-methyl guanosine derivative (mG), titration of the imino proton spectra and chemical shift perturbations were performed on hZαADAR1 upon binding to Z-DNA. The structural transition between B-Z conformation as the changing ratio between DNA and protein showed a binding affinity of the modified DNA onto the Z-DNA binding protein similar to wild-type DNA or RNA. The chemical shift perturbation results showed that the overall structure and environment of the modified DNA revealed DNA-like properties rather than RNA-like characteristics. Moreover, we found evidence for two distinct regimes, "Z-DNA Sensing" and "Modification Sensing", based on the site-specific chemical shift perturbation between the DNA (or RNA) binding complex and the modified DNA-hZαADAR1 complex. Thus, we propose that modification of the sugar backbone of DNA with 2'-O-methyl guanosine promotes the changes in the surrounding α3 helical structural segment as well as the non-perturbed feature of the β-hairpin region. Copyright © 2020 Elsevier Inc. All rights reserved.

Citation

Ho-Seong Jin, Na-Hyun Kim, Seo-Ree Choi, Kwang-Im Oh, Joon-Hwa Lee. Protein-induced B-Z transition of DNA duplex containing a 2'-OMe guanosine. Biochemical and biophysical research communications. 2020 Dec 10;533(3):417-423