Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1.

The interaction between neurons and glia is pivotal for the development of chronic opioid tolerance. One of the most important mechanisms of cell-to-cell interaction is the Notch signaling pathway. In this chapter we propose a double-immunofluorescence method to observe and quantify the colocalization of Notch-1 and mu-opioid receptor (MOR-1), using both neuronal and astrocyte markers.

Citation

Vittoria Borgonetti, Nicoletta Galeotti. Fluorescence Colocalization Analysis of Cellular Distribution of MOR-1. Methods in molecular biology (Clifton, N.J.). 2021;2201:27-34

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PMID: 32975786

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