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The versatility of Clustered Regularly Interspaced Short Palindromic Repeats/Cas (CRISPR/Cas) genome editing tool ushered biologists into an exciting era of editing genomes with great efficiency and at a pace that was never imagined before. Though the CRISPR/Cas genome editing was developed after Zinc Finger Nucleases (ZFNs) and Transcription activator-like effector nucleases (TALENs), it is more popular and successful than these genome editing systems. The advent of targetable nucleases such as Cas9 has enabled manipulation of genomes in an accurate and precise manner. The CRISPR/Cas system of editing plant genomes has technical and economical advantages over conventional breeding methods. It has led to the development of traits within plant genomes that fulfill the needs of mankind. Advent of innovative procedures have paved the way for effective and efficient genome editing that has revolutionized genetic aspects and meets the safety regulations toward development of crops. The present review highlights the critical aspects of employing CRISPR/Cas for editing plant genomes in comparison with previously known editing approaches, such as ZFNs and TALENs. The study includes descriptive information on the approaches, procedural programs and applications in editing plant genomes for improving traits such as crop yield, resistance against emerging pathogens, abiotic stresses and herbicide tolerance thereof in the present-day world. Copyright © 2020 Elsevier B.V. All rights reserved.


Mujtaba Aamir Bhat, Mudasir Ahmad Bhat, Vijay Kumar, Ishfaq Ahmad Wani, Humayra Bashir, Ali Asghar Shah, Safikur Rahman, Arif Tasleem Jan. The era of editing plant genomes using CRISPR/Cas: A critical appraisal. Journal of biotechnology. 2020 Dec 20;324:34-60

PMID: 32980369

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