GTPases Arf5 and Arl2 function partially distinctly during oocyte meiosis.

Mammalian female meiosis must be tightly regulated to produce high-quality mature oocytes for subsequent regular fertilization and healthy live birth of the next generation. GTPases control many important signal pathways involved in diverse cellular activities. ADP-ribosylation factor family members (Arfs) in mice possess GTPase activities, and some members have been found to function in meiosis. However, whether other Arfs play a role in meiosis is unknown. In this study, we found that Arl2 and Arf5 are the richest among Arfs in mouse oocytes, and they are more abundant in oocytes than in granular cells. Furthermore, Arl2 and Arf5 depletion both impeded meiotic progression, but by affecting spindles and microfilaments, respectively. Moreover, Arl2 and Arf5 depletion both significantly increased regular reactive oxygen species levels and decreased mitochondrial membrane potential and autophagy, indicating that oocyte quality was damaged by Arl2 and Arf5 depletion. These results suggest that Arl2 and Arf5 are two novel essential GTPases required for oocyte meiosis and quality control. © 2020 Wiley Periodicals LLC.

Citation

Chun-Xiang Zhou, Yang Wang, Li-Ya Shi, Zi-Bin Wang, Yang Ma, Cong-Rong Li, Na-Na Zhang, Ye-Xiao Zhang, Fenli Zhang, Dong Zhang, Zheng-Rong Xia. GTPases Arf5 and Arl2 function partially distinctly during oocyte meiosis. Journal of cellular biochemistry. 2021 Feb;122(2):198-208

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PMID: 32985032

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