New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii.

Valeria Blanda, Rosalia D'Agostino, Elisabetta Giudice, Kety Randazzo, Francesco La Russa, Sara Villari, Stefano Vullo, Alessandra Torina

Molecules (Basel, Switzerland) 2020 Sep 27

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Rickettsia species are an important cause of emerging infectious diseases in people and animals, and rickettsiosis is one of the oldest known vector-borne diseases. Laboratory diagnosis of Rickettsia is complex and time-consuming. This study was aimed at developing two quantitative real-time PCRs targeting ompB and ompA genes for the detection, respectively, of Rickettsia spp. and R. conorii DNA. Primers were designed following an analysis of Rickettsia gene sequences. The assays were optimized using SYBR Green and TaqMan methods and tested for sensitivity and specificity. This study allowed the development of powerful diagnostic methods, able to detect and quantify Rickettsia spp. DNA and differentiate R. conorii species.

Citation

Valeria Blanda, Rosalia D'Agostino, Elisabetta Giudice, Kety Randazzo, Francesco La Russa, Sara Villari, Stefano Vullo, Alessandra Torina. New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii. Molecules (Basel, Switzerland). 2020 Sep 27;25(19)