CbCYP716A261, a New β-Amyrin 28-Hydroxylase Involved in Conyzasaponin Biosynthesis from Conyza blinii].

R Sun, J L Gao, H Chen, S Liu, Z Z Tang

Molekuliarnaia biologiia 2020 Sep-Oct

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Conyzasaponins produced by the traditional Chinese herb Conyza blinii are oleanane-type saponins with a wide range of biological activities. Here, we identified a gene, designated CbCYP716A261, encoding a β-amyrin 28-hydroxylase in conyzasaponins biosynthesis. Ten full putative CYP sequences were isolated from Conyza blinii transcript tags. The CbCYP716A261 gene product was selected as the putative β-amyrin 28-hydroxylase by phylogenetic analysis and transcriptional activity analysis of methyl jasmonate-treated Conyza blinii. To identify the enzymatic activity, we performed enzymatic activity experiments in vitro and in vivo. The HPLC results revealed that CbCYP716A261 catalyzes the hydroxylation of β-amyrin at the C-28 position to yield oleanolic acid. Our findings provide new information about the conyzasaponin biosynthesis pathway and widen the list of isolated β-amyrin 28-hydroxylases.

Citation

R Sun, J L Gao, H Chen, S Liu, Z Z Tang. CbCYP716A261, a New β-Amyrin 28-Hydroxylase Involved in Conyzasaponin Biosynthesis from Conyza blinii]. Molekuliarnaia biologiia. 2020 Sep-Oct;54(5):813-825