Yutaro Kitanaka, Yasuo Takeuchi, Koichi Hiratsuka, Nay Aung, Yuriko Sakamaki, Takashi Nemoto, Walter Meinzer, Yuichi Izumi, Takanori Iwata, Akira Aoki
Photodiagnosis and photodynamic therapy 2020 DecThis study aimed to investigate the effects of a new antimicrobial photodynamic therapy (aPDT) system using yellow-green light-emitting diode (YGL) and rose bengal (RB) on Porphyromonas gingivalis (Pg) in vitro. Pg suspension mixed with RB was irradiated with YGL (565 nm) or blue light-emitting diode (BL, 470 nm) at 428 mW/cm2 in comparison with chlorhexidine (CHG) treatment. The cells were cultured anaerobically on agar plates, and the number of colony-forming units (CFU) was determined. The treated suspension was anaerobically incubated, and the cell density (OD600nm) was monitored for 24 h. Also, the viability of treated human gingival fibroblast (HGF-1) was measured using WST-8 assay. Pg morphology was observed with a scanning electron microscope. The RNA integrity number of aPDT-treated Pg was determined and gene expressions were evaluated by quantitative real-time polymerase chain reaction. RB + YGL (aPDT) demonstrated a significantly higher reduction of CFU, compared to RB + BL (aPDT) and CHG, furthermore the OD value rapidly decreased. Morphological changes of Pg with RB + YGL were more severe than with CHG. Although RB + YGL reduced HGF-1 viability, aPDT's impact was significantly lower than CHG's. With RB + YGL treatment, RIN values decreased; furthermore, gene expressions associated with DNA replication and cell division were remarkably decreased after 12 h. The results of this study demonstrated that a novel aPDT system using RB + YGL may have potential as a new technical modality for bacterial elimination in periodontal therapy. Copyright © 2020 Elsevier B.V. All rights reserved.
Yutaro Kitanaka, Yasuo Takeuchi, Koichi Hiratsuka, Nay Aung, Yuriko Sakamaki, Takashi Nemoto, Walter Meinzer, Yuichi Izumi, Takanori Iwata, Akira Aoki. The effect of antimicrobial photodynamic therapy using yellow-green LED and rose bengal on Porphyromonas gingivalis. Photodiagnosis and photodynamic therapy. 2020 Dec;32:102033
PMID: 33011393
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