Correlation Engine 2.0
Clear Search sequence regions


  • bacteria (1)
  • FLP (3)
  • gcd (1)
  • gene protein (1)
  • glf (1)
  • glk (1)
  • gluconates (2)
  • Sizes of these terms reflect their relevance to your search.

    Komagataeibacter xylinus has received increasing attention as an important microorganism for the conversion of several carbon sources to bacterial cellulose (BC). However, BC productivity has been impeded by the lack of efficient genetic engineering techniques. In this study, a lambda Red and FLP/FRT-mediated site-specific recombination system was successfully established in Komagataeibacter xylinus. Using this system, the membrane bound gene gcd, a gene that encodes glucose dehydrogenase, was knocked out to reduce the modification of glucose to gluconic acid. The engineered strain could not produce any gluconic acid and presented a decreased bacterial cellulose (BC) production due to its restricted glucose utilization. To address this problem, the gene of glucose facilitator protein (glf; ZMO0366) was introduced into the knockout strain coupled with the overexpression of the endogenous glucokinase gene (glk). The BC yield of the resultant strain increased by 63.63-173.68%, thus reducing the production cost.

    Citation

    Ling-Pu Liu, Xue Yang, Xiang-Jun Zhao, Kai-Yue Zhang, Wen-Chao Li, Yan-Yan Xie, Shi-Ru Jia, Cheng Zhong. A Lambda Red and FLP/FRT-Mediated Site-Specific Recombination System in Komagataeibacter xylinus and Its Application to Enhance the Productivity of Bacterial Cellulose. ACS synthetic biology. 2020 Nov 20;9(11):3171-3180

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 33048520

    View Full Text