Ginnosuke Yamaguchi, Shiori Habara, Shotaro Suzuki, Yuki Ugachi, Hisashi Kawai, Takuro Nakajima, Munetaka Shimizu
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 2021 JanIn this study, we examined the effects of porcine growth hormone (GH) and cortisol on plasma insulin-like growth factor binding proteins (IGFBPs) in juveniles of three subspecies of Oncorhynchus masou (masu, amago, and Biwa salmon). Ligand blotting using digoxigenin-labeled human IGF-I was used to detect and semi-quantify three major circulating IGFBP bands at 41, 28, and 22 kDa, corresponding to IGFBP-2b, -1a, and -1b, respectively. GH increased plasma IGFBP-2b concentration in masu and Biwa salmon but suppressed it in amago salmon. Plasma IGFBP-2b levels were increased by cortisol in the three subspecies. Cortisol induced plasma IGFBP-1a in the three subspecies, whereas GH had a suppressive effect in masu and Biwa salmon. Sham and cortisol injections increased plasma IGFBP-1b levels after 1 day in masu and amago salmon, suggesting that IGFBP-1b is induced following exposure to stressors via cortisol. Increased IGFBP-1b levels were restored to basal levels when co-injected with GH in Biwa salmon, and the same trend was seen in masu and amago salmon. However, the suppressive effect of GH disappeared 2 days after injection in the three subspecies. Despite some differences among subspecies, the findings suggest that cortisol is a primary inducer of plasma IGFBP-1b; however, GH counteracts it in the short term. Therefore, GH has the potential to modulate the degree of increase in circulating IGFBP-1b levels during acute stress. Copyright © 2020 Elsevier Inc. All rights reserved.
Ginnosuke Yamaguchi, Shiori Habara, Shotaro Suzuki, Yuki Ugachi, Hisashi Kawai, Takuro Nakajima, Munetaka Shimizu. Effects of growth hormone and cortisol administration on plasma insulin-like growth factor binding proteins in juveniles of three subspecies of masu salmon (Oncorhynchus masou). Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 2021 Jan;251:110821
PMID: 33053436
View Full Text