Effect of Methionine on AMD1 Gene Expression in Prostate Cancer Cells.

Shaochen Shen, Linhong Zeng, Hongwei Huang

Nutrition and cancer 2021

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To investigate the effect of AMD1 gene expression on prostate cancer cells (PC-3M), explore the mechanism of AMD1 action in cancer cells, and examine the regulation of AMD1 gene expression by methionine (MET). Quantitative PCR (qPCR) and western blot analysis (WB) approaches were used to detect and measure gene expression. The cell apoptotic rate was determined by flow cytometric (FCM) analysis. qPCR and WB assays showed that both AMD1 gene expression and cell apoptotic rate were associated with MET. MET has a significant regulatory effect on the expression of the AMD1 gene and a certain amount of MET can promote the expression of the AMD1 gene. This provides a health guideline for a low-methionine diet for prostate cancer patients and scientific evidence for prostate cancer prevention.

Citation

Shaochen Shen, Linhong Zeng, Hongwei Huang. Effect of Methionine on AMD1 Gene Expression in Prostate Cancer Cells. Nutrition and cancer. 2021;73(9):1804-1815