Probing gold nanoparticles for the desensitization to β-lactoglobulin from binding mechanism, structure and IgE binding changes.

Xiaoning Zhang, Meifeng Li, Yuanping Lv, Xiaoling Sun, Yao Han, Bing Liu, Xiangzhong Zhao, Xiaowen Huang

Food chemistry 2021 Apr 16

filter terms:

Because of the adsorption of proteins, gold nanoparticles (AuNPs) create potential biological risks in biomedicine, leading to the formation of the protein corona. This adsorption is mainly due to the formation of gold-sulfur (AuS) covalent bonds between the AuNPs and the -SH groups, causing bioactivity denaturation and biological problems; however, it could also lead to some biological benefits. We explored AuNPs as a potential material for desensitization to allergens, such as β-lactoglobulin (βLG). To address the desensitization of AuNPs, we investigated the binding mechanism and the specific relationship of the time evolution of AuS bond, secondary structure, and allergy changes. The formation of AuS bond takes approximately 9 h, consistent with the complete changes time in secondary structure and immunoglobulin E (IgE) combining capacity of the βLG, decreasing allergic reactions. These results indicate that AuNPs have the potential to minimize allergic reactions in the future. Copyright © 2020 Elsevier Ltd. All rights reserved.

Citation

Xiaoning Zhang, Meifeng Li, Yuanping Lv, Xiaoling Sun, Yao Han, Bing Liu, Xiangzhong Zhao, Xiaowen Huang. Probing gold nanoparticles for the desensitization to β-lactoglobulin from binding mechanism, structure and IgE binding changes. Food chemistry. 2021 Apr 16;342:128329