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Cultivation of Chlamydia species in cell lines requires centrifugation of the inoculum onto diethylaminoethyl-dextran-pretreated cell monolayers to improve the infection efficiency. Here we report that the addition of DNA transfection reagent Lipofectamine in the inoculum significantly enhances the infectivity of Chlamydia abortus in mouse fibroblast McCoy cells, with an infection efficiency equivalent to that of the centrifugation method. Similar enhancement effects of Lipofectamine on the infectivity of C. psittaci and C. trachomatis were also observed. This study provides an alternative and convenient method for the cultivation of Chlamydia species in vitro in the absence of centrifugation. Copyright © 2020 Elsevier Inc. All rights reserved.

Citation

Zhaocai Li, Ping Liu, Jizhang Zhou. Lipofectamine enhances Chlamydia infectivity in cell culture. Analytical biochemistry. 2020 Dec 01;610:113985

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PMID: 33065117

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